DSS-induced animals are weighed daily, and body weight change as compared to Day 0 is calculated. The AUC is calculated to compare treatment arms and is shown in the inset. (**p<0.01; ***p<0.005 compared to the vehicle-control).

DSS-induced animals are assessed daily for weight loss, diarrhea, and blood in stool phenotypes. Each phenotype is scored and used to calculate the composite Disease Activity Index score. The AUC is calculated to compare treatment arms and is shown in the inset. (***p<0.005 compared to the vehicle-control).

DSS-induced animals are assessed for colonic inflammation and ulceration by video endoscopy and are scored for colitis severity (left) and stool consistency (right). (*p<0.05; ****p<0.001 compared to the vehicle-control).

Following euthanasia, the colon is excised, measured, rinsed, and weighed; the colon weight:length ratio is calculated. (****p<0.001 compared to the vehicle-control).

H&E stained slides from mouse colon tissue are evaluated with light microscopy by an ACVP board-certified veterinary pathologist (Dallas Tissue Research). Features of inflammation, necrosis/gland loss, erosions, submucosal edema, and epithelial hyperplasia are given an individual score and scores are added together to obtain a sum score. Naïve is shown in upper left and diseased is shown in lower left. (****p<0.001).

Colon samples are digested for leukocyte enrichment, stained, and are assessed via Flow Cytometry for neutrophils. (*p<0.05).

Spleen and mLN samples are dissociated, stained, and assessed via Flow Cytometry for neutrophils. Analysis for additional cell types is available. (**p<0.01; ****p<.0001).

Colon samples are homogenized and assessed via multiplex cytokine analysis for IFNγ, IL-1β, IL-6, IL-17, KC, and TNFα. Additional cytokines are available.