The rIL-2 induced vascular leak syndrome (VLS) model is often used to recapitulate IL-2 toxicity associated with immunotherapy. Manifestations of disease include transendothelial migration of inflammatory cells into the lung and liver which leads to organ injury. In rodents, rIL2 elicits a strong immune response within hours following administration. This response is measured by examining lung and liver vascular permeability by Evan’s blue assays, and complete blood cell (CBC) counts in blood.
Following rIL-2 administration, Evans Blue dye localization in the lungs is assessed by measuring the absorbance at 650nm in the tissue. (*p<0.05; **p<0.01 as compared to vehicle control). Representative images of the lungs from each group are shown.
Following rIL-2 administration, Evans Blue dye localization is in the liver is assessed by measuring the absorbance at 650nm in the tissue. (**p<0.01 as compared to vehicle control). Representative images of the liver from each group are shown.
Following rIL-2 administration, blood is collected upon euthanasia and Complete Blood Cell Count is performed. Mean group values for WBC and Lymphocyte counts are shown. (*p<0.05; **p<0.01, ****p<0.0001 as compared to vehicle control)
