Differential response to TNBS-induced colitis induction can be observed in animals pre-treated with antibiotic, likely due to perturbation or reduction of the constitutive microbiome. Depending on the antibiotic pre-treatment paradigm selected, evaluation of colitis phenotype in antibiotic pre-treated animals enables assessment of phenotypes’ sensitivity toward broad microbiome depletion, species-specific depletion, or function-specific depletion. We can work with you to share our experience with antibiotic-induced microbiome modulation and how these treatments may impact your study goals.

Data from the acute TNBS model induced in animals pre-treated with an antibiotic cocktail intended for maximum microbiome depletion is shown.

• C57Bl/6 Mice

• Percent weight change
• Survival
• Endoscopy: colitis severity and stool consistency scoring
• Terminal colon weight and length measurement

• Disease Activity Index: diarrhea and blood in stool scoring
• Terminal histopathology and/or IHC analyses
• Colon cytokines
• Colon myeloperoxidase (MPO)
• Fecal LCN2, calprotectin, & MPO
• Bacterial load (culture/qPCR)
• Sequencing and metagenomics
• Peripheral lymphoid organ immunophenotyping
• Intestinal lamina propria immunophenotyping
• Custom assays