Differential responses in tumor growth can be observed in animals sourced from different vendors, some of which are mediated by vendor-differences in microbiome. Evaluation of tumor growth and checkpoint inhibitor sensitivity in animals sourced from different vendors can be a straightforward approach to assess the microbiome’s impact on these phenotypes. Data from a syngeneic subcutaneous melanoma model induced in animals from different vendors is shown.

• C57Bl/6 Mice

• Tumor measurement
• Body condition scoring

• Assessment of bacteria load (culture and qPCR)
• Sequencing and metagenomics
• Peripheral lymphoid organ immunophenotyping (Flow Cytometry)
• Tumor infiltrating lymphocyte immunophenotyping (Flow Cytometry)
• Custom assays