Differential response to syngeneic tumor growth can be observed in germ-free animals relative to those with an endogenous microbiome. Evaluation of tumor growth and checkpoint inhibitor sensitivity in germ-free mice enables assessment of phenotypes’ dependency on a constitutive microbiome, whether due to the microbiome itself or due to other immunological effects. Data from a syngeneic subcutaneous melanoma model induced in germ-free mice is shown.

• C57Bl/6 Mice

• Tumor measurement
• Body condition scoring

• Assessment of bacteria load (culture and qPCR)
• Sequencing and metagenomics
• Peripheral lymphoid organ immunophenotyping (Flow Cytometry)
• Tumor infiltrating lymphocyte immunophenotyping (Flow Cytometry)
• Custom assays