S. typhimurium-Induced Colitis

Description

BioModels offers a S. typhimurium-induced colitis model. Salmonella enterica is a gram-negative, facultative anaerobic pathogen that can impact the gastrointestinal systems of both human and animal populations. S. enterica subspecies 1 serovar Typhimurium is the primary etiological agent of foodborne salmonellosis resulting in non-systemic enterocolitis in humans. Oral infection of susceptible C57Bl/6 mice with serovar Typhimurium is an established in vivo model used to study the pathogenesis of Salmonella-induced gastroenteritis and to assess the efficacy of novel therapeutics. This infection is characterized by weight loss, epithelial barrier disruption, and increased inflammation in the gastrointestinal tract. Representative data demonstrates kinetics of disease severity over 72 hours with body weight tracking, assessment of colonic infiltrating neutrophils with correlating MPO production, and histopathology analysis of colon segments.

  • Advantages: translational animal model for infectious gastroenteritis, established intestinal epithelial barrier disruption, natural human pathogen.
  • Disadvantages: requires pre-treatment with streptomycin.
Model Systems
  • C57Bl/6 Mice
Standard Readouts
  • Percent weight change
  • Terminal histopathology and/or IHC analyses
  • Colonic immunophenotyping
  • Colonic myeloperoxidase (MPO)
Additional Readouts
  • Survival
  • Intestinal and systemic bacterial burden
  • Disease Activity Index: diarrhea and blood in stool scoring
  • Terminal colon weight and length measurement
  • Endoscopy
  • Colon cytokines
  • Peripheral lymphoid organ immunophenotyping
  • Intestinal lamina propria immunophenotyping
  • Custom assays
Representative DataClick Image to Enlarge
Percent Weight Change in a Murine Model of S. typhimurium-Induced Infectious Colitis

Animals are weighed daily, and percent body weight change relative to Day 0 is calculated. AUCs are calculated to compare infected and uninfected animals and are shown in the figure inset (***p<0.001).

Colonic Luminal Neutrophils and Fecal Myeloperoxidase Levels in a Murine Model of S. typhimurium-Induced Infectious Colitis

Colons and fecal pellets are collected from infected animals. Colon tissue is processed and analyzed via FACS for neutrophil populations. Fecal homogenates are assessed for myeloperoxidase levels. Infected groups were compared to naïve animals (*p<0.05; **p<0.01).

Colon Histopathology Scoring in a Murine Model of S. typhimurium-Induced Infectious Colitis

Colons of infected animals at t=24, 48, and 72 hours are compared with naïve colons via histopathology analysis (*p<0.05; **p<0.01).

Colon Histopathology from a Murine Model of S. typhimurium-Induced Infectious Colitis

Representative H&E stained colon samples from infected animals at t=24, 48, and 72hrs compared to a naïve colon. Black arrows denote inflammation. Blue arrows denote edema. Black brackets denote mucosal necrosis. Histopathology performed by Dallas Tissue Research.

Daniel Lichtman

Managing Partner