Hydrochloric Acid-Induced Lung Injury

Description

BioModels offers a hydrochloric acid (HCl)-induced acute lung injury (ALI) mouse model. In the HCl-ALI model, mice are anesthetized and then administered HCl by the intratracheal route. Animals are evaluated daily for body weight and respiratory status. Endpoints in the model are most often measured 24 hours following HCl administration. Endpoints in this model include total and differential cell counts and inflammatory mediator content in the broncho-alveolar lavage fluid, detailed lung mechanics, as well as histopathology and immunohistochemistry.

Model Systems
  • BALB/c Mice
Standard Readouts
  • Percent weight change
  • BAL total and differential cell counts
  • BAL total protein
  • Lung mechanics
  • BAL fluid and lung cytokines
  • Terminal histopathology and/or IHC analyses
Additional Readouts
  • Peripheral lymphoid organ immunophenotyping
  • Lung immunophenotyping
  • Custom assays
Representative DataClick Image to Enlarge
Dose Response of Inflammatory Cells in BAL fluid from HCl-induced ALI model

BALB/c mice are administered HCl on Day 0 and BAL fluid is assessed at 24 hours for total neutrophils and total protein content following administration of either 50 µL, 62.5 µL, or 75 µL of HCl.

Pressure Volume Curves and Tissue Elastance from HCl-induced ALI

BALB/c mice are administered HCl on Day 0 and lung mechanics are assessed at 24 hours following administration of either 50 µL, 62.5 µL, or 75 µL of HCl.

Daniel Lichtman

Managing Partner