Microbiome and RA – GPI- Induced Arthritis – Vendor Comparison

Description

Differential response to arthritis induction can be observed in animals sourced from different vendors, some of which are mediated by vendor-differences in microbiome. Evaluation of Rheumatoid Arthritis (RA) phenotypes in animals sourced from different vendors can be a straightforward approach to assess the microbiome’s impact in a given model. Data from the Glucose-6-phosphate isomerase (GPI)-induced arthritis model induced in animals from different vendors is shown as an example.

Model Systems
  • DBA/1
Standard Readouts
  • Percent weight change
  • Arthritis score
  • Disease incidence
  • Paw thickness
Additional Readouts
  • Plasma/serum cytokines
  • Terminal histopathology and/or IHC analyses
  • Peripheral lymphoid organ immunophenotyping
  • Bacterial load (culture/qPCR)
  • Sequencing and metagenomics
  • Custom assays
Representative DataClick Image to Enlarge
Rheumatoid Arthritis Scores in the Glucose-6-phosphate Isomerase (GPI) Model of Arthritis

Animals are monitored for signs of arthritis on a daily basis and assigned RA scores. The AUC is calculated to compare treatment arms and is shown in the inset. (*p<0.05).

Disease Incidence the Glucose-6-phosphate Isomerase (GPI) Model of Arthritis

Animals are monitored for signs of arthritis on a daily basis and overall disease incidence is shown.

Daniel Lichtman

Managing Partner