Differential responses to metabolic disease can be observed in animals pre-treated with antibiotic, likely due to perturbation or reduction of the constitutive microbiome. Depending on the antibiotic pre-treatment paradigm selected, evaluation of obesity and metabolism phenotype in antibiotic pre-treated animals enables assessment of phenotypes’ sensitivity toward broad microbiome depletion, species-specific depletion, or function-specific depletion. Data assessing metabolic phenotypes in normal mice, as well as from the high fat diet-induced obesity model, utilizing animals pre-treated with an antibiotic cocktail intended for maximum microbiome depletion is shown. A subset of animals were treated with a murine fecal microbial transplant (FMT).

• C57Bl/6 Mice

• Percent weight change
• Body composition via DEXA: bone mineral density & content, bone area, lean & fat mass, body fat percentage
• Blood glucose

• Oral glucose tolerance test
• Terminal histopathology and/or IHC analyses
• Peripheral cytokine analysis
• Serum chemistry analysis
• Serum triglyceride and cholesterol analysis
• Peripheral lymphoid organ immunophenotyping
• Bacterial load (culture/qPCR)
• Sequencing and metagenomics
• Custom assays