LPS-Induced Lung Injury

Description

BioModels offers an LPS-induced acute lung injury (ALI) model. In the LPS-ALI model, mice are anesthetized and then administered LPS by the intranasal route. Animals are evaluated daily for body weight and respiratory status. Endpoints in the model are most often measured between 4-96 hours following LPS administration, with peak disease typically observed around 48 hours. Endpoints in this model include total and differential cell counts and inflammatory mediator content in the broncho-alveolar lavage fluid, detailed lung mechanics, as well as histopathology and immunohistochemistry.

Model Systems
  • BALB/c Mice
Standard Readouts
  • Percent weight change
  • BAL total and differential cell counts
  • BAL total protein
  • Lung mechanics
  • BAL fluid and lung cytokines
  • Terminal histopathology and/or IHC analyses
Additional Readouts
  • Peripheral lymphoid organ immunophenotyping
  • Lung immunophenotyping
  • Custom assays
Representative DataClick Image to Enlarge
Images of BAL fluid from LPS-induced ALI

BALB/c mice are administered LPS on Day 0 and BAL fluid is assessed at 24 hours.

Dose Response of Inflammatory Cells in BAL fluid from LPS-induced ALI model

BALB/c mice are administered LPS on Day 0 and BAL fluid is assessed at 24 hours for total cells and total neutrophils following administration of either 1 µg or 10 µg of LPS.

Images of H&E-stained fixed lung tissue from LPS-induced ALI

BALB/c mice are administered LPS on Day 0 and lung tissue is collected at 24 hours following administration of either 1 µg or 10 µg of LPS.

Time Course of Inflammatory Response in BAL fluid from LPS-induced ALI model

BALB/c mice are administered LPS on Day 0 and BAL fluid is assessed at 48 or 96 hours for total cells, total neutrophils, total protein, and cytokines following administration 10 µg of LPS. (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001 compared to the naïve control).

Time Course of Systemic Inflammatory Response in Plasma from LPS-induced ALI model

BALB/c mice are administered LPS on Day 0 and plasma is assessed at 48 or 96 hours for cytokines following administration 10 µg of LPS. (*p<0.05; **p<0.01; ***p<0.001 compared to the naïve control).

Percent Weight Change in the LPS-induced ALI model

BALB/c mice are administered LPS on Day 0 and weight change is assessed. Animals are weighed daily, and body weight change as compared to Day 0 is calculated. The AUC is calculated to compare treatment arms and is shown in the inset.

Images of H&E-stained fixed lung tissue from LPS-induced ALI with Dexamethasone Treatment

BALB/c mice are administered LPS on Day 0 and lung tissue is collected at 48 hours following administration of either 10 µg of LPS or 10 µg of LPS with Dexamethasone treatment.

Pressure Volume Curves from LPS-induced ALI with Dexamethasone Treatment

BALB/c mice are administered LPS on Day 0 and lung mechanics are assessed at 48 hours following administration of either 10 µg of LPS or 10 µg of LPS with Dexamethasone treatment.

Lung Mechanics and Inflammatory Response in BAL Fluid from LPS-induced ALI with Dexamethasone Treatment

BALB/c mice are administered LPS on Day 0 and lung elastance and BAL fluid total cells, total neutrophils, and cytokines are assessed at 48 hours following administration 10 µg of LPS.

Daniel Lichtman

Managing Partner