In the fractionated radiation-induced dermal fibrosis model, the back of the mouse is shaved and/or depilated and the skin is temporarily tented such that it can be selectively targeted with radiation while the rest of the mouse is protected by a lead shield. Animals receive 6 fractions of radiation on a schedule of 3 days on, 2 days off. The progression of disease, which can also be observed in an acute radiation model, displays with mild erythema typically observed 8-10 days following radiation exposure, with dermatitis severity peaking between Days 12-16 (depending on the dose of radiation), and with desquamation of approximately 50% of the irradiated area. Peak levels of fibrosis are observed at 36 days following the start of radiation. Endpoints include dermatitis severity scores, collagen content measurement, and histological analyses.

• BALB/c Mice

• Percent weight change
• Terminal histopathology and/or IHC analyses
• Skin collagen content

• Dermatitis severity scores
• Systemic and tissue cytokine analysis
• Immunophenotyping
• Custom assays