Differential response to DSS-induced colitis induction can be observed in animals sourced from different vendors, some of which are mediated by differences in their microbiome. Evaluation of colitis phenotypes in animals sourced from different vendors can be a straightforward approach to assess the microbiome’s impact in a given colitis model. Data from the acute DSS model induced in animals from different vendors is shown. When utilizing the DSS-induced colitis model for assessment of novel therapeutics, we can help you select the most appropriate animal vendor to ensure the right microbiome for your experimental needs.

• C57Bl/6 Mice

• Percent weight change
• Endoscopy: colitis severity and stool consistency scoring
• Terminal colon weight and length measurement

• Disease Activity Index: diarrhea and blood in stool scoring
• Terminal histopathology and/or IHC analyses
• Colon cytokines
• Colon myeloperoxidase (MPO)
• Fecal LCN2, calprotectin, & MPO
• Peripheral lymphoid organ immunophenotyping
• Intestinal lamina propria immunophenotyping
• Custom assays
• Bacterial Load (culture/PCR)
• Sequencing and metagenomics