Diet Induced Obesity (DIO)

Description

Macronutrients of rodent chow can be manipulated to introduce specific levels of dietary fat. Rodents placed on a diet that provides at least 30% of total daily energy intake from fat provide a translational preclinical model of obesity (western diet). These models can be used to identify specific mechanistic factors that are contributing to diet-induced obesity and to assess novel therapeutics (e.g- GLP-1 agonists) for their potential in treating this metabolic disorder. BioModels can provide the expertise necessary to design and execute a diet induced obesity model that is customized to meet your specific needs with flexible experimental endpoints.

Model Systems
  • C57Bl/6 Mice
  • Zucker Rats
Standard Readouts
  • Percent weight change
  • Bone densitometry (DEXA)
  • Body composition (DEXA)
Additional Readouts
  • Wound healing
  • Blood glucose
  • Blood insulin
  • Glucose or Insulin tolerance testing (GTT or ITT)
  • Histopathology and/or IHC analyses
  • Multiplex/cytokine analysis
  • Peripheral lymphoid organ immunophenotyping
  • Custom assays
Representative DataClick Image to Enlarge
Percent Weight Change in a Murine Model of High Fat Diet-Induced Obesity

Animals maintained on a control or high-fat diet are weight daily, and percent weight change as compared to Day 0 is calculated. The AUC is calculated to compare treatment arms and is shown in the inset. (***p<0.001).

Bone Density Measurements in a Murine Model of High Fat Diet-Induced Obesity

Animals maintained on a control or high-fat diet underwent a dual-energy X-ray absorptiometry (DEXA) scan to assess bone density, bone mineral content, and bone area. (**p<0.01).

Body Composition Measurements in a Murine Model of High Fat Diet-Induced Obesity

Animals maintained on a control or high-fat diet underwent a dual-energy X-ray absorptiometry (DEXA) scan to assess lean tissue mass, fat tissue mass, and percent body fat. (***p<0.001; ****p<0.0001).

Daniel Lichtman

Managing Partner