Diet-Induced NASH (AMLN/GAN)

Description

Feeding mice an Amylin liver NASH (AMLN) diet leads to obesity, fatty liver, and after longer amounts of time, both liver inflammation and fibrosis. Animals maintained on this diet are a widely used model for nonalcoholic steatohepatitis (NASH).  This model is often referred to as the Gubran Amylin NASH (GAN) diet of obesity/NASH.

Model Systems
  • C57Bl/6 Mice maintained on AMLN diet
Standard Readouts
  • Histopathology and/or IHC analyses
  • Percent weight change
  • Blood glucose
  • Blood insulin
  • Liver weight
  • Liver collagen content
  • Body composition analysis (DEXA)
  • Blood chemistry
Additional Readouts
  • Multiplex/cytokine analysis
  • Peripheral lymphoid organ Immunophenotyping
  • Custom assays
Representative DataClick Image to Enlarge
Liver Histopathology and Collagen Content in the Murine Model of Diet-Induced NASH

Liver samples from control and NASH diet-fed animals (either 40 or 53 weeks on diet) are processed for histopathology for determination of NAFLD Activity Score (H&E) and collagen content (PSR).  Sum of steatosis grade (0-3), lobular inflammation (0-3), and ballooning degeneration (0-2) was calculated with a total range of 0-8 to determine NAFLD Activity Score. Central vein (C) and portal tract (P) are indicated. Macrovesicular (black arrow) and microvesicular (black arrowhead) steatosis are indicated.  Percent collagen (PSR) positive area was calculated by taking the quotient of total positive area (um2) over the total area of the hepatic ROI (um2). (**p<0.01 compared to the control diet group). Histopathology performed by Dallas Tissue Research.

Body Composition Measurements in the Murine Model of Diet-Induced NASH

NASH diet-fed animals (either 40 or 53 weeks on diet) and control-diet fed animals had body composition analysis performed using a DEXA Scanner.  Increases in Fat and Total Tissue are observed in NASH Diet-fed animals (*p<0.05 compared to the control diet group).

Serum Chemistry in the Murine Model of Diet-Induced NASH

NASH diet-fed animals (either 40 or 53 weeks on diet) and control-diet fed animals had blood collected and assessed for levels of Alanine transaminase (ALT), Aspartate transaminase (AST), and Alkaline.

Daniel Lichtman

Managing Partner