Diabetic Wound Healing (Splinted & Non-Splinted)

Description

There are several well characterized, genetic rodent preclinical models of spontaneous Type I and Type II diabetes including the Zucker fatty rat (T1D), the ZDF rat (T2D), the ob/ob mouse (T2D), and the db/db mouse (T2D) that recapitulate autoimmune aspects of the human disease. Often, animals with these genetic backgrounds display delays in wound healing.  Both splinted and non-splinted wound healing models are available. Splinted models allow you to test your treatment in the absence of wound contraction that is often observed in rodents.  BioModels has the expertise and experience to help you select the model that will be most appropriate for your goals of testing novel therapeutics, assessing specific mechanisms of the metabolic disorder, or addressing certain medical complications that arise in diabetic patients.

Model Systems
  • Zucker rats
  • ZDF rats
  • ob/ob mice
  • db/db mice
Standard Readouts
  • Percent weight change
  • Blood glucose
  • Blood insulin
  • Wound Closure Measurements
Additional Readouts
  • Survival
  • Histopathology and/or IHC analyses
  • Multiplex/cytokine analysis
  • Peripheral lymphoid organ immunophenotyping
  • Custom assays
Representative DataClick Image to Enlarge
Percent Weight Change in a Murine Splinted Wound Healing Model

Animals with splinted wounds are weighed daily and percent body weight change relative to Day 0 is calculated. The AUC is calculated to compare groups and is shown in the inset. (****p<0.0001).

Blood Glucose Levels in a Murine Splinted Wound Healing Model

Pre-study and terminal blood glucose levels were measured via glucose monitor. (****p<0.0001).

Percent Wound Open in a Murine Splinted Wound Healing Model

Wound healing is measured with calipers three times per week during study. The AUC is calculated to compare groups and is shown in the inset. (***p<0.001).

Daniel Lichtman

Managing Partner